Coding

Part:BBa_K1039013:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-11)


PhiC31 Recombination Directionality Factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 195
    Illegal SapI site found at 417
    Illegal SapI site found at 543


Design Notes

Added base after the stop codon to prevent creation of an SpeI site due to addition of suffix


Source

The sequence was obtained by Genbank and was synthesized by BioBasic Inc

References